Application of fluorescent and UV-Vis detection methods to profile antimicrobial activity of cassava tissues for an efficient Agrobacterium-mediated transformation.
Identifieur interne : 000134 ( Main/Exploration ); précédent : 000133; suivant : 000135Application of fluorescent and UV-Vis detection methods to profile antimicrobial activity of cassava tissues for an efficient Agrobacterium-mediated transformation.
Auteurs : Bella Rhea Lavifa Sanjaya [Indonésie, Japon] ; Sholeh Avivi [Indonésie] ; Tri Agus Siswoyo [Indonésie] ; Ara Most Tanziman [Japon] ; Shinjiro Ogita [Japon]Source :
- Plant biotechnology (Tokyo, Japan) [ 1342-4580 ] ; 2019.
Abstract
The majority of tissue culture and transformation studies in cassava (Manihot esculenta Crantz) focus on the modification of conditions in order to establish a better protocol. Although this is a standard approach for making progress in genetic transformation technology for a target plant variety, serious difficulty still remains due to the limited applicability and adaptability of the given protocol. In the present study, we aim to develop a new concept that focuses on the development of simple but adaptable genetic transformation technology in cassava. In order to establish an efficient transformation protocol, two local edible cassava varieties, R-type, with a broad leaf with reddish petiole, and S-type, with a thin leaf with shiny greenish petiole, were obtained from Okinawa, Japan. Three detection methods, i.e., fluorescence microscopy, thin-layer chromatography (TLC) with detection under an ultraviolet (UV) illumination (254 nm) and light emitting diode (LED) illuminations (365 nm and 500 nm) without any staining, and a spectrum scanning (250-700 nm) by a microplate reader system were employed to identify a series of unique features of the petioles and leaves. Antimicrobial activity of methanol extracts from the tissues was also assayed. We succeeded in the transient expression of the GUS gene using cassava leaves and also established stable introduction of the GUS gene into three organogenic cassava calli by adapting Agrobacterium-mediated transformation. With all the findings, we have identified a flexible tool to create a better match between explants and Agrobacterium strains.
DOI: 10.5511/plantbiotechnology.19.0203a
PubMed: 31275051
PubMed Central: PMC6566009
Affiliations:
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Three detection methods, i.e., fluorescence microscopy, thin-layer chromatography (TLC) with detection under an ultraviolet (UV) illumination (254 nm) and light emitting diode (LED) illuminations (365 nm and 500 nm) without any staining, and a spectrum scanning (250-700 nm) by a microplate reader system were employed to identify a series of unique features of the petioles and leaves. Antimicrobial activity of methanol extracts from the tissues was also assayed. We succeeded in the transient expression of the <i>GUS</i> gene using cassava leaves and also established stable introduction of the <i>GUS</i> gene into three organogenic cassava calli by adapting <i>Agrobacterium</i>-mediated transformation. With all the findings, we have identified a flexible tool to create a better match between explants and <i>Agrobacterium</i> strains.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31275051</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1342-4580</ISSN><JournalIssue CitedMedium="Print"><Volume>36</Volume><Issue>1</Issue><PubDate><Year>2019</Year></PubDate></JournalIssue><Title>Plant biotechnology (Tokyo, Japan)</Title><ISOAbbreviation>Plant Biotechnol (Tokyo)</ISOAbbreviation></Journal><ArticleTitle>Application of fluorescent and UV-Vis detection methods to profile antimicrobial activity of cassava tissues for an efficient <i>Agrobacterium</i>-mediated transformation.</ArticleTitle><Pagination><MedlinePgn>57-61</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.5511/plantbiotechnology.19.0203a</ELocationID><Abstract><AbstractText>The majority of tissue culture and transformation studies in cassava (<i>Manihot esculenta</i> Crantz) focus on the modification of conditions in order to establish a better protocol. Although this is a standard approach for making progress in genetic transformation technology for a target plant variety, serious difficulty still remains due to the limited applicability and adaptability of the given protocol. In the present study, we aim to develop a new concept that focuses on the development of simple but adaptable genetic transformation technology in cassava. In order to establish an efficient transformation protocol, two local edible cassava varieties, R-type, with a broad leaf with reddish petiole, and S-type, with a thin leaf with shiny greenish petiole, were obtained from Okinawa, Japan. Three detection methods, i.e., fluorescence microscopy, thin-layer chromatography (TLC) with detection under an ultraviolet (UV) illumination (254 nm) and light emitting diode (LED) illuminations (365 nm and 500 nm) without any staining, and a spectrum scanning (250-700 nm) by a microplate reader system were employed to identify a series of unique features of the petioles and leaves. Antimicrobial activity of methanol extracts from the tissues was also assayed. We succeeded in the transient expression of the <i>GUS</i> gene using cassava leaves and also established stable introduction of the <i>GUS</i> gene into three organogenic cassava calli by adapting <i>Agrobacterium</i>-mediated transformation. 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IdType="pubmed">31275051</ArticleId><ArticleId IdType="doi">10.5511/plantbiotechnology.19.0203a</ArticleId><ArticleId IdType="pmc">PMC6566009</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Plant Res. 2009 Jul;122(4):455-63</Citation><ArticleIdList><ArticleId IdType="pubmed">19308313</ArticleId></ArticleIdList></Reference><Reference><Citation>J Integr Plant Biol. 2011 Jul;53(7):552-69</Citation><ArticleIdList><ArticleId IdType="pubmed">21564542</ArticleId></ArticleIdList></Reference><Reference><Citation>GM Crops. 2011 Jun-Dec;2(3):193-200</Citation><ArticleIdList><ArticleId IdType="pubmed">22179195</ArticleId></ArticleIdList></Reference><Reference><Citation>In Vitro Cell Dev Biol Plant. 2016;52(5):461-478</Citation><ArticleIdList><ArticleId IdType="pubmed">27818605</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2017 Aug 14;12(8):e0180736</Citation><ArticleIdList><ArticleId 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Rhea Lavifa Sanjaya</name></noRegion><name sortKey="Ogita, Shinjiro" sort="Ogita, Shinjiro" uniqKey="Ogita S" first="Shinjiro" last="Ogita">Shinjiro Ogita</name><name sortKey="Tanziman, Ara Most" sort="Tanziman, Ara Most" uniqKey="Tanziman A" first="Ara Most" last="Tanziman">Ara Most Tanziman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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